Nanomechanics and Raman Spectroscopy of in Situ Native Carbohydrate Storage Granules for Enhancing Starch Quality and Lignocellulosic Biomass Production.
Identifieur interne : 000253 ( Main/Exploration ); précédent : 000252; suivant : 000254Nanomechanics and Raman Spectroscopy of in Situ Native Carbohydrate Storage Granules for Enhancing Starch Quality and Lignocellulosic Biomass Production.
Auteurs : Rubye H. Farahi [États-Unis] ; Aude L. Lereu [France] ; Anne M. Charrier [France] ; Udaya C. Kalluri [États-Unis] ; Brian H. Davison [États-Unis] ; Ali Passian [États-Unis]Source :
- ACS omega [ 2470-1343 ] ; 2020.
Abstract
Alternative energy strategies based on plant biomass-derived bioenergy and biofuels rely on understanding and optimization of plant structure, chemistry, and performance. Starch, a constitutive element of all green plants, is important to food, biofuels, and industrial applications. Models of carbohydrate storage granules are highly heterogeneous in representing morphology and structure, though a deeper understanding of the role of structure in functional behavior is emerging. A better understanding of the in situ nanoscale properties of native granules is needed to help improve the starch quality in food crops as well as optimize lignocellulosic biomass production in perennial nonfood crops. Here, we present a new technique called soft mechanical nano-ablation (sMNA) for accessing the interior of the granules without compromising the inner nanostructure. We then explore the nanomechanics of granules within the ray parenchyma cells of Populus xylem, a desirable woody biofuel feedstock. The employed soft outer layer nanoablation and atomic force microscopy reveal that the inner structure comprises 156 nm blocklets arranged in a semicrystalline organization. The nanomechanical properties of the inner and outer structures of a single starch granule are measured and found to exhibit large variations, changing by a factor of 3 in Young's modulus and a factor of 2 in viscoplastic index. These findings demonstrate how the introduced approach facilitates studies of structure-function relationships among starch granules and more complex secondary cell wall features as they relate to plant performance.
DOI: 10.1021/acsomega.9b02849
PubMed: 32095683
PubMed Central: PMC7033668
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nanomechanics and Raman Spectroscopy of in Situ Native Carbohydrate Storage Granules for Enhancing Starch Quality and Lignocellulosic Biomass Production.</title><author><name sortKey="Farahi, Rubye H" sort="Farahi, Rubye H" uniqKey="Farahi R" first="Rubye H" last="Farahi">Rubye H. Farahi</name><affiliation wicri:level="1"><nlm:affiliation>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation></author><author><name sortKey="Lereu, Aude L" sort="Lereu, Aude L" uniqKey="Lereu A" first="Aude L" last="Lereu">Aude L. Lereu</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille University, CNRS, Centrale Marseille, Institut Fresnel, Marseille 13397, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille University, CNRS, Centrale Marseille, Institut Fresnel, Marseille 13397</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Charrier, Anne M" sort="Charrier, Anne M" uniqKey="Charrier A" first="Anne M" last="Charrier">Anne M. Charrier</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille University, CNRS, CINaM, Marseille 13288, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille University, CNRS, CINaM, Marseille 13288</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation></author><author><name sortKey="Davison, Brian H" sort="Davison, Brian H" uniqKey="Davison B" first="Brian H" last="Davison">Brian H. Davison</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996</wicri:regionArea><wicri:noRegion>Tennessee 37996</wicri:noRegion></affiliation></author><author><name sortKey="Passian, Ali" sort="Passian, Ali" uniqKey="Passian A" first="Ali" last="Passian">Ali Passian</name><affiliation wicri:level="1"><nlm:affiliation>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996</wicri:regionArea><wicri:noRegion>Tennessee 37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Physics, University of Tennessee, Knoxville, Tennessee 37996, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physics, University of Tennessee, Knoxville, Tennessee 37996</wicri:regionArea><wicri:noRegion>Tennessee 37996</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32095683</idno><idno type="pmid">32095683</idno><idno type="doi">10.1021/acsomega.9b02849</idno><idno type="pmc">PMC7033668</idno><idno type="wicri:Area/Main/Corpus">000434</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000434</idno><idno type="wicri:Area/Main/Curation">000434</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000434</idno><idno type="wicri:Area/Main/Exploration">000434</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Nanomechanics and Raman Spectroscopy of in Situ Native Carbohydrate Storage Granules for Enhancing Starch Quality and Lignocellulosic Biomass Production.</title><author><name sortKey="Farahi, Rubye H" sort="Farahi, Rubye H" uniqKey="Farahi R" first="Rubye H" last="Farahi">Rubye H. Farahi</name><affiliation wicri:level="1"><nlm:affiliation>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation></author><author><name sortKey="Lereu, Aude L" sort="Lereu, Aude L" uniqKey="Lereu A" first="Aude L" last="Lereu">Aude L. Lereu</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille University, CNRS, Centrale Marseille, Institut Fresnel, Marseille 13397, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille University, CNRS, Centrale Marseille, Institut Fresnel, Marseille 13397</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Charrier, Anne M" sort="Charrier, Anne M" uniqKey="Charrier A" first="Anne M" last="Charrier">Anne M. Charrier</name><affiliation wicri:level="3"><nlm:affiliation>Aix Marseille University, CNRS, CINaM, Marseille 13288, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix Marseille University, CNRS, CINaM, Marseille 13288</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName></affiliation></author><author><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation></author><author><name sortKey="Davison, Brian H" sort="Davison, Brian H" uniqKey="Davison B" first="Brian H" last="Davison">Brian H. Davison</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996</wicri:regionArea><wicri:noRegion>Tennessee 37996</wicri:noRegion></affiliation></author><author><name sortKey="Passian, Ali" sort="Passian, Ali" uniqKey="Passian A" first="Ali" last="Passian">Ali Passian</name><affiliation wicri:level="1"><nlm:affiliation>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830</wicri:regionArea><wicri:noRegion>Tennessee 37830</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996</wicri:regionArea><wicri:noRegion>Tennessee 37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Physics, University of Tennessee, Knoxville, Tennessee 37996, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physics, University of Tennessee, Knoxville, Tennessee 37996</wicri:regionArea><wicri:noRegion>Tennessee 37996</wicri:noRegion></affiliation></author></analytic><series><title level="j">ACS omega</title><idno type="eISSN">2470-1343</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Alternative energy strategies based on plant biomass-derived bioenergy and biofuels rely on understanding and optimization of plant structure, chemistry, and performance. Starch, a constitutive element of all green plants, is important to food, biofuels, and industrial applications. Models of carbohydrate storage granules are highly heterogeneous in representing morphology and structure, though a deeper understanding of the role of structure in functional behavior is emerging. A better understanding of the in situ nanoscale properties of native granules is needed to help improve the starch quality in food crops as well as optimize lignocellulosic biomass production in perennial nonfood crops. Here, we present a new technique called soft mechanical nano-ablation (sMNA) for accessing the interior of the granules without compromising the inner nanostructure. We then explore the nanomechanics of granules within the ray parenchyma cells of <i>Populus</i> xylem, a desirable woody biofuel feedstock. The employed soft outer layer nanoablation and atomic force microscopy reveal that the inner structure comprises 156 nm blocklets arranged in a semicrystalline organization. The nanomechanical properties of the inner and outer structures of a single starch granule are measured and found to exhibit large variations, changing by a factor of 3 in Young's modulus and a factor of 2 in viscoplastic index. These findings demonstrate how the introduced approach facilitates studies of structure-function relationships among starch granules and more complex secondary cell wall features as they relate to plant performance.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32095683</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">2470-1343</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Feb</Month><Day>18</Day></PubDate></JournalIssue><Title>ACS omega</Title><ISOAbbreviation>ACS Omega</ISOAbbreviation></Journal><ArticleTitle>Nanomechanics and Raman Spectroscopy of in Situ Native Carbohydrate Storage Granules for Enhancing Starch Quality and Lignocellulosic Biomass Production.</ArticleTitle><Pagination><MedlinePgn>2594-2602</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acsomega.9b02849</ELocationID><Abstract><AbstractText>Alternative energy strategies based on plant biomass-derived bioenergy and biofuels rely on understanding and optimization of plant structure, chemistry, and performance. Starch, a constitutive element of all green plants, is important to food, biofuels, and industrial applications. Models of carbohydrate storage granules are highly heterogeneous in representing morphology and structure, though a deeper understanding of the role of structure in functional behavior is emerging. A better understanding of the in situ nanoscale properties of native granules is needed to help improve the starch quality in food crops as well as optimize lignocellulosic biomass production in perennial nonfood crops. Here, we present a new technique called soft mechanical nano-ablation (sMNA) for accessing the interior of the granules without compromising the inner nanostructure. We then explore the nanomechanics of granules within the ray parenchyma cells of <i>Populus</i> xylem, a desirable woody biofuel feedstock. The employed soft outer layer nanoablation and atomic force microscopy reveal that the inner structure comprises 156 nm blocklets arranged in a semicrystalline organization. The nanomechanical properties of the inner and outer structures of a single starch granule are measured and found to exhibit large variations, changing by a factor of 3 in Young's modulus and a factor of 2 in viscoplastic index. These findings demonstrate how the introduced approach facilitates studies of structure-function relationships among starch granules and more complex secondary cell wall features as they relate to plant performance.</AbstractText><CopyrightInformation>Copyright © 2020 American Chemical Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Farahi</LastName><ForeName>Rubye H</ForeName><Initials>RH</Initials><AffiliationInfo><Affiliation>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lereu</LastName><ForeName>Aude L</ForeName><Initials>AL</Initials><AffiliationInfo><Affiliation>Aix Marseille University, CNRS, Centrale Marseille, Institut Fresnel, Marseille 13397, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Charrier</LastName><ForeName>Anne M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Aix Marseille University, CNRS, CINaM, Marseille 13288, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kalluri</LastName><ForeName>Udaya C</ForeName><Initials>UC</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davison</LastName><ForeName>Brian H</ForeName><Initials>BH</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Passian</LastName><ForeName>Ali</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Computational Sciences and Engineering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Physics, University of Tennessee, Knoxville, Tennessee 37996, United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Omega</MedlineTA><NlmUniqueID>101691658</NlmUniqueID><ISSNLinking>2470-1343</ISSNLinking></MedlineJournalInfo><CoiStatement>The authors declare no competing financial interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32095683</ArticleId><ArticleId IdType="doi">10.1021/acsomega.9b02849</ArticleId><ArticleId IdType="pmc">PMC7033668</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Agric Food Chem. 2002 Jul 3;50(14):3912-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12083858</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomacromolecules. 2010 May 10;11(5):1139-53</Citation><ArticleIdList><ArticleId IdType="pubmed">20405913</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2017 Jul 20;68(16):4433-4453</Citation><ArticleIdList><ArticleId IdType="pubmed">28981786</ArticleId></ArticleIdList></Reference><Reference><Citation>Carbohydr Res. 2001 Jan 30;330(2):249-56</Citation><ArticleIdList><ArticleId IdType="pubmed">11217978</ArticleId></ArticleIdList></Reference><Reference><Citation>Biomacromolecules. 2003 Sep-Oct;4(5):1198-202</Citation><ArticleIdList><ArticleId IdType="pubmed">12959584</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2008 Oct;28(10):1593-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18708341</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Biol Macromol. 1997 Aug;21(1-2):103-7</Citation><ArticleIdList><ArticleId IdType="pubmed">9283023</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2015 Oct;239:1-8</Citation><ArticleIdList><ArticleId IdType="pubmed">26398785</ArticleId></ArticleIdList></Reference><Reference><Citation>Carbohydr Polym. 2014 Mar 15;103:355-8</Citation><ArticleIdList><ArticleId IdType="pubmed">24528740</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Biol Macromol. 1998 Aug;23(2):85-112</Citation><ArticleIdList><ArticleId IdType="pubmed">9730163</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Dec;7(1):152</Citation><ArticleIdList><ArticleId IdType="pubmed">28273953</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2016 Jul;73(14):2781-807</Citation><ArticleIdList><ArticleId IdType="pubmed">27166931</ArticleId></ArticleIdList></Reference><Reference><Citation>Carbohydr Res. 1973 Jul;29(1):123-34</Citation><ArticleIdList><ArticleId IdType="pubmed">4751262</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Environ. 2009 Dec;32(12):1724-36</Citation><ArticleIdList><ArticleId IdType="pubmed">19671097</ArticleId></ArticleIdList></Reference><Reference><Citation>Ultramicroscopy. 2003 Oct-Nov;97(1-4):151-69</Citation><ArticleIdList><ArticleId IdType="pubmed">12801668</ArticleId></ArticleIdList></Reference><Reference><Citation>Physiol Plant. 2014 Aug;151(4):480-94</Citation><ArticleIdList><ArticleId IdType="pubmed">24320774</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1986 Sep;168(3):377-80</Citation><ArticleIdList><ArticleId IdType="pubmed">24232147</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Bioanal Chem. 2010 Aug;397(7):2693-701</Citation><ArticleIdList><ArticleId IdType="pubmed">20213166</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Bot. 2010 Dec;106(6):885-95</Citation><ArticleIdList><ArticleId IdType="pubmed">21037242</ArticleId></ArticleIdList></Reference><Reference><Citation>Spectrochim Acta A Mol Biomol Spectrosc. 2017 Oct 5;185:317-335</Citation><ArticleIdList><ArticleId IdType="pubmed">28599236</ArticleId></ArticleIdList></Reference><Reference><Citation>Crit Rev Food Sci Nutr. 2015;55(8):1081-97</Citation><ArticleIdList><ArticleId IdType="pubmed">24915341</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Opt. 1995 Jan 1;34(1):201-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20963105</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Farahi, Rubye H" sort="Farahi, Rubye H" uniqKey="Farahi R" first="Rubye H" last="Farahi">Rubye H. Farahi</name></noRegion><name sortKey="Davison, Brian H" sort="Davison, Brian H" uniqKey="Davison B" first="Brian H" last="Davison">Brian H. Davison</name><name sortKey="Davison, Brian H" sort="Davison, Brian H" uniqKey="Davison B" first="Brian H" last="Davison">Brian H. Davison</name><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><name sortKey="Passian, Ali" sort="Passian, Ali" uniqKey="Passian A" first="Ali" last="Passian">Ali Passian</name><name sortKey="Passian, Ali" sort="Passian, Ali" uniqKey="Passian A" first="Ali" last="Passian">Ali Passian</name><name sortKey="Passian, Ali" sort="Passian, Ali" uniqKey="Passian A" first="Ali" last="Passian">Ali Passian</name><name sortKey="Passian, Ali" sort="Passian, Ali" uniqKey="Passian A" first="Ali" last="Passian">Ali Passian</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Lereu, Aude L" sort="Lereu, Aude L" uniqKey="Lereu A" first="Aude L" last="Lereu">Aude L. Lereu</name></region><name sortKey="Charrier, Anne M" sort="Charrier, Anne M" uniqKey="Charrier A" first="Anne M" last="Charrier">Anne M. Charrier</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000253 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000253 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32095683
|texte= Nanomechanics and Raman Spectroscopy of in Situ Native Carbohydrate Storage Granules for Enhancing Starch Quality and Lignocellulosic Biomass Production.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32095683" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |